Functional Activity of Virus Antibodies in Immune Globulins: West Nile Virus, for example
نویسنده
چکیده
.................................................................................................. 1 Zusammenfassung ..................................................................................... 2 2. West Nile Virus Introduction ............................................................... 4 1.1. Virology ............................................................................................ 4 1.2. Epidemiology ..................................................................................... 5 1.3. Clinical Manifestation and Diagnosis ..................................................... 8 1.4. Treatment and IGIV ......................................................................... 10 1.5. IgG subclasses ................................................................................ 11 3. Materials and Methods........................................................................ 14 3.1. Test articles .................................................................................... 14 3.2. Virus and cells ................................................................................. 14 3.3. IgG subclass separation .................................................................... 15 3.3.1. FPLC program ................................................................................ 15 3.3.2. ÄKTA program(1) ........................................................................... 17 3.3.3. ÄKTA program(2) ........................................................................... 19 3.4. Elisa ............................................................................................... 20 3.5. RID ................................................................................................ 22 3.6. HPLC .............................................................................................. 24 3.7. WNV infectivity and neutralization test ............................................... 25 3.7.1. TCID50 assay .................................................................................. 26 3.7.2. WNV micro neutralization assay ....................................................... 26 3.8. In vivo protection against WNV challenge ............................................ 28 -
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